PCR DNA PRODUCT PURIFICATION |
|
Last Update: December 2006 |
|
Based on the kits developed by QIAGEN |
|
|
|
| PROCEDURE | |
| 1. Add 5 volumes of Buffer PB to 1 volume of the PCR reaction and mix | |
| 2. Place a QIAquick spin column in a 2 mL tube | |
| 3. To bind DNA, apply sample to column and centrofuge for 30-60 secs | |
4. Discard FT and plave column back into the tube |
|
| 5. Wash with 0.75 mL of Buffer PE and centrifuge for 30-60 secs | |
| 6. Discard FT. Place column back in tube and centrifuge for an additional 1 min | |
| 7. Place column in a clean 1.5 mL tube | |
| 8. Add 30-50 mL of Buffer EB or dH2O, let stand for 1 min, and centrifuge for 1 min | |
The DNA is ready for use. |
|